Pathways Analysis and Design for Fatty Acid Synthesis

การวิเคราะห์และออกแบบวิถีการสังเคราะห์กรดไขมัน

Name: Chompunuch Liamprawat

Name: ชมพูนุช เลี่ยมประวัติ

Organization : King Mongkut's University of Technology Thonburi. Faculty of Engineering. Chemical Engineering

keyword: กรดไขมัน

Abstract: Fatty acid has many advantages in term of medication, pharmacy and nutrition etc. However, the utilization of fatty acid is still high due to the production from the current source is not enough to satisfy the market demand. This research lntended to identify new alternative fatty acid source by studying in Baker's yeast because of having the complete genome data. Note that the metabolic network from the existing strain has produced the fatty acid in lower level. This research was aimed to analyze and design metabolic network in yeast, S.ceuevisiae, to increase higher oleic acid synthesis. The metabolic network of the existing yeast collected from journals, biochemical textbooks and lnternet database was constructed and simplified to represent the fatty acid synthesis pathways in yeast. Then, the stoicheometric material balance incorporating 44 reaction equations, 44-enzyme directionality and 54 metabolites was distinguished. These were used for analyze and design yeast's network to increase fatty acid synthesis by Elementary Flux Analysis (EFA) with METATOOL program. This technique was able to define the possible simple ways as EFMs (Elementary Flux Mode) which is starting from glucose converted to oleic acid. Knowledge of EFM was useful for determining optimal and suboptimal theoretical conversion of oleic acid from glucose. From results, the optimal and suboptimal theoritical yield of oleic acid were 0.222, 0.185 and 0.1 11 mole/molegl,,,,, respectively. These EFMs guild us possible approach for to analysis and design of new pathways which can increase synthesize fatty acid yield. Application of mutant in S.cerevisiae was used to be the other tool to assist designing increased fatty acid synthesis pathways. The EFA interpretation results implied that deleating glucose 6-phosphate dehydrogenase (gpd) and glucosephosphophase isomer enzyme (gpi) out of the existing pathways could increase fatty acid yield. In addition, this research was studied the entirely cellular metabolism in yeast with EFA technique was studied for determining the possible optimal theoretical ylelds products of interest such as amino acid, fatty acid and macrsmolecules be as benchmarks in Laboratory.

King Mongkut's University Technology Thonburi. Library

Address: Bangkok

Email: info.lib@mail.kmutt.ac.th

Name: Asawin Meechai

Role: Advisor

Name: Sakarindr Bhumiratana

Role: Advisor

Created: 2001

Issued: 2007-12-27

Modified: 2015-11-25

วิทยานิพนธ์/Thesis

CallNumber: CHE1225

eng

DegreeName: Master of Engineering

Level: Master's Degree

Descipline: Chemical Engineering

Grantor: King Mongkut's University of Technology Thonburi

©copyrights King Mongkut's University of Technology Thonburi

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1	CHE1225.pdf	3.14 MB	141	2018-11-17 11:12:05
2	CHE1225ab.pdf	177.61 KB	49	2018-05-10 18:50:29
3	CHE1225ab.txt	2.47 KB	29	2018-11-10 06:24:43

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