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Sirisuda Pontumma. Screen-printed electrode for the deterction of pesticides: Effect of water-miscible organic solvents on the activity of enzymes and the inhibition by organophosphate pesticides. Master's Degree(Biochemical Technology). King Mongkut's University Technology Thonburi. Library. : King Mongkut's University of Technology Thonburi, 2004.
Screen-printed electrode for the deterction of pesticides: Effect of water-miscible organic solvents on the activity of enzymes and the inhibition by organophosphate pesticides
การพัฒนาเซนเซอร์แบบพิมพ์สกรีนสำหรับตรวจวัดยาปราบศัตรูพืช: ผลของตัวทำละลายอินทรีย์ชนิดละลายน้ำ (water-miscible) ต่อกิจกรรมของเอนไซม์และการยับยั้งกิจกรรมของเอนไซม์ โดยยาปราบศัตรูพืชกลุ่มออร์กาโนฟอสเฟต
Name: Sirisuda Pontumma
Organization :
King Mongkut's University of Technology Thonburi. School of Bioresources and Technology. Biochemical Technology
keyword:
Biosensors
Abstract:
The work described in this research concerns the development of organophosphatebiosensor working in water-miscible solvents. The aspects include the production ofscreen printed electrodes suitable for measurement in organic solvents-buffer mixtures.The activity of AChE and ChOX has been studied via reduction of the releasedhydrogen peroxide at rhodium-on-carbon working electrodes at 0.0 mV vs. AgIAgCl.Ethanol, acetone, acetonitrile and methanol were used in this investigation. Screenprinted electrodes was also used to assess the effect of organic solvents on the inhibitoryeffect of paraoxon on AChE activity. An increase in current response was observedfrom 0.5-10% (vlv) ethanol and acetonitrile, while the enzymatic activity slightlyincreased in acetone-buffer mixtures at concentration of 10%. At the highest percentageof methanol (lo%), 24% loss of enzyme activity was obtained. The inhibitory capacityof AChE was maintained in all four organic solvents with different percentage ofinhibitions. The highest sensitivity to paraoxon was found for combination of 0.005 unitAChE and 1 unit ChOX. The biosensor was operated in the mixture of 10% ethanol andphosphate buffer, and the incubation time for exposing in paraoxon was 30 min. withthe optimized procedure described, the detection limit for paraoxon of the biosensor was0.1 ppm.
King Mongkut's University Technology Thonburi. Library
Address:
กรุงเทพมหานคร (Bangkok)
Email:
info.lib@mail.kmutt.ac.th
Name: Pornpimol Sritongkham
Role:
Advisor
Name: Werasak Surareungchai
Role:
Advisor
Created:
2004
Issued:
2007-06-12
Modified:
2007-07-19
วิทยานิพนธ์/Thesis
ISBN:
9741841272
CallNumber:
BCT103
eng
DegreeName: Master of Science
Level: Master's Degree
Descipline: Biochemical Technology
©copyrights King Mongkut's University of Technology Thonburi
RightsAccess:
ใช้เวลา
0.038141 วินาที
Sirisuda Pontumma
| Title | Contributor | Type |
|---|---|---|
| Screen-printed electrode for the deterction of pesticides: Effect of water-miscible organic solvents on the activity of enzymes and the inhibition by organophosphate pesticides
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี Sirisuda Pontumma | Pornpimol Sritongkham Werasak Surareungchai | วิทยานิพนธ์/Thesis |
Pornpimol Sritongkham
| Title | Creator | Type and Date Create |
|---|---|---|
| Screen-printed electrode for the deterction of pesticides: Effect of water-miscible organic solvents on the activity of enzymes and the inhibition by organophosphate pesticides
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี Pornpimol Sritongkham;Werasak Surareungchai | Sirisuda Pontumma | วิทยานิพนธ์/Thesis |
| Amperometric biosensor based on poly (3,4-ethylenedioxythiophene) / poly(styrenesulfonate) / gold nanocomposite modified screen-printed carbon electrode for triglyceride determination
จุฬาลงกรณ์มหาวิทยาลัย ;Seeroong Prichanont;Pornpimol Sritongkham | Angkana Phongphut | วิทยานิพนธ์/Thesis |
Werasak Surareungchai