Sasithorn Kongruang. Optimization of oleaginous yeast production by central composite design. (). King Mongkut's University of Technology North Bangkok. Central Library. : , 2018.
Optimization of oleaginous yeast production by central composite design
Abstract:
Rhodosporidium toruloides is one of the high potential oleaginous yeasts for the
application in food additives, pharmaceuticals and feed ingredients. The effect of growth
parameters to biomass and lipid production of R.toruloides TISTR 5149 in molasses media
was studied and optimized by response surface methodology via Central Composite Design.
Fermentation parameters affecting the lipid production using 40, 50 and 60 g/L molasses as
carbon source, pH 5, 6 and 7, temperatures at 28, 30 and 32 °C and shaking speeds at 200,
250 and 300 rpm were investigated for biomass and lipid production. Results found that
biomass and lipid production can be explained using the quadratic models. Results also
revealed that biomass yielded 15.47 g/L under 50 g/L molasses, 200 rpm , 32 °C, pH 5.0
while pH and shaking speed were significantly effect to lipid production (p = 0.0015 and
0.0006) where the highest lipid production was 11.88 g/L. Models were validated and showed
a good agreement to predict biomass but not lipid production. This mathematical relationship
of biomass production can be applied to scale up in pilot scale.
King Mongkut's University of Technology North Bangkok. Central Library
Address:
BANGKOK
Email:
library@kmutnb.ac.th
Created:
2018
Modified:
2562-06-27
Issued:
2019-06-27
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BibliograpyCitation :
In Prince of Songkla University Faculty of Science. Pure and Applied Chemistry International Conference (PACCON 2018) (p.EE1-EE6). Songkla : Prince of Songkla University