Capillary Gas Chromatography Determination of Positional Selectivity of Lipase

Name: Narumon Jeyashoke

Organization : King Mongkut's Institute of Technology Thonburi. School of Bioresources and Technology. Department of Biotechnology

Name: Kanit Krisnangkura

Organization : King Mongkut's Institute of Technology Thonburi. School of Bioresources and Technology. Department of Biotechnology

keyword: Lipase

Abstract: Lipase catalyzed hydrolysis of fats and oils may show positional selectivity or may be non-selective. Positional selectivity of lipase is determined by using tripalmitin as a substrate. Dipalmitoylglycerol formed in the reaction were sparated and quantified by capillary gas chromatography (GC) as their trimethylsilyl ethers. Candida cylindracea lipase which is positionally non-selective can hydrolyze C-1 or C-3 ester linkage of tripalmitin at a rate twice that at the C-2 position. Although lipases from pancreatic, Aspergillus niger, Rhizopus arrhizus and Rhizopus delemar are considered 1.3-selective, they also can attack the C-2 position of tripalmitin. The hydrolytic rate, however, at the C-2 position is much slower than at the C-1 position. A positional selectivity index (PSI) for a given lipase is proposed as a measure of relative rate of hydrolysis of C-1 (c-3) and C-2 positions of tripalmitin

King Mongkut's Institute of Technology Thonburi

Address: Bangkok

Email: info.lib@mail.kmutt.ac.th

Created: 1994

Issued: 2005-11-24

Modified: 2553-02-10

บทความ/Article

BibliograpyCitation : KMITT Research and Development Journal vol. 17, no. 1 (Apr. 1994), p. 54-62

CallNumber: 12000578

eng

©copyrights King Mongkut's University of Technology Thonburi

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