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Suriya Jansaengsri. Utilization of tissue culture technique for propagation of Melia spp.. Master's Degree(Environmental Biology). Mahidol University. Mahidol University Library and Knowledge Center. : Mahidol University, 1992.
Utilization of tissue culture technique for propagation of Melia spp.
การใช้เทคนิคการเพาะเลี้ยงเนื้อเยื่อการขยายพันธุ์ไม้เลื่ยน
Name: Suriya Jansaengsri
LCSH:
Tissue culture
Abstract:
Tissue culture technique applied for mass propagation of Melia spp. is possible. The modified LS basal medium used in this study is composed of macronutrients, micronutrients from LS medium, vitamins of B5 proposed by Gamborg, 6 g/l agar and 10% coconut water. The explants used in this experiment were young leaf, petiole, internode, terminal bud and axillary bud from young plant. The optimun concentration of BA for multiple shoot induction from axillary bud and terminal bud was 1 mg/l. Callus cultures can be readily initiated and proliferated from petiole and internode in the basal medium supplemented with 1 mg/l NAA plus 1 mg/l BA but no callus mass was observed from young leaf explant. The derived callus cultured on basal medium supplemented with 1 mg/l BA regenerated shoots which had a vigorous growth in basal medium with 0.25 regale BA. The individual shootlets regenerated vigorous roots within 7 - 10 days after immersion of the basal cut end into the solution of 1 mg/l IAA and 1 mg/l IBA for 1 day, then transferral to basal medium; 85% of the treated shoots rooted. One month old plantlets were then transplanted into pots filled with sterilized vermiculite under high humidity conditions. The survival percentage was about 21%, most of the mortality being due to fungus infection.
Mahidol University. Mahidol University Library and Knowledge Center
Address:
NAKHONPATHOM
Email:
liwww@mahidol.ac.th
Name: Sarinee Chaicharoen
Role:
Thesis Advisors
Name: Visut Baimai
Role:
Thesis Advisors
Name: Thatchai Umprai
Role:
Thesis Advisors
Created:
1992
Modified:
2018-02-23
Issued:
2018-02-23
วิทยานิพนธ์/Thesis
application/pdf
eng
DegreeName: Master of Science
Level: Master's Degree
Descipline: Environmental Biology
Grantor: Mahidol University
ใช้เวลา
0.259449 วินาที
Suriya Jansaengsri
| Title | Contributor | Type |
|---|---|---|
| Utilization of tissue culture technique for propagation of Melia spp.
มหาวิทยาลัยมหิดล Suriya Jansaengsri | Sarinee Chaicharoen Visut Baimai Thatchai Umprai | วิทยานิพนธ์/Thesis |
Sarinee Chaicharoen
| Title | Creator | Type and Date Create |
|---|---|---|
| Studies on seed germination and plant growth development of Thai wild orchid, aerides houlletiana and acampe rigida
มหาวิทยาลัยมหิดล Sarinee Chaicharoen;Maleeya Kruatrachue;Suchart Upatham | Karnchana Rungruchkanont | วิทยานิพนธ์/Thesis |
| Utilization of tissue culture technique for propagation of Melia spp.
มหาวิทยาลัยมหิดล Sarinee Chaicharoen;Visut Baimai;Thatchai Umprai | Suriya Jansaengsri | วิทยานิพนธ์/Thesis |
| In vitro induction of polyploid in white mulberry (Moru alba var. $54) by colchicine treatment
มหาวิทยาลัยมหิดล Sarinee Chaicharoen;Maleya Kruatrachue;Ajaraporn Sailasuta | Ariya Satrabhandhu | วิทยานิพนธ์/Thesis |
Visut Baimai
Thatchai Umprai
| Title | Creator | Type and Date Create |
|---|---|---|
| Utilization of tissue culture technique for propagation of Melia spp.
มหาวิทยาลัยมหิดล Sarinee Chaicharoen;Visut Baimai;Thatchai Umprai | Suriya Jansaengsri | วิทยานิพนธ์/Thesis |