Abstract:
Eighteen strains of ectomycorrhizal fungi were isolated from root of Pine(Pinus spp.) seedlings. Pine seedlings were collected from nursery which locate in five provinces of Thailand. Grouping them into five groups based on the similarity of their colony and mycelium formation pattern. Isolated ectomycorrhizal fungi Surin 1, Pisanulok 2, Saraburi 3, Tak 4 and Ubolrachathani 3 were represented to Group 1,2,3,4 and 5 respectively. The inoculum medium containing 1:50 volume by volume of organic soil (soil plow) and vermiculite gave the highest growth rate in all five representative isolated ectomycorrhizal fungi. Comparing growth rate of infected khasia pine seedling with five representative isolated ectomycorrhizal fungi and uninfected khasia pine seedlttgs found that five months old infected khasia pine seedling with all five isolated ectomycorrhizal fungi showed kigher growth rate statistically significance than uninfected khasia pine seedlings.