Study on the reactivity of dengue virus specific proteins with patients' sera

การศึกษาปฏิกิริยา อิมมูโนวิทยาระหว่างโปรตีน แอนติเจนของแดงกี่ไวรัสกับซีรั่มของผู้ป่วยไข้เลือดออก

Name: Tasanee Mongkolsuk

MeSH: Dengue Virus

MeSH: Fluorescent Antibody Technique

MeSH: Hemorrhagic Fevers, Viral

Abstract: One hundred and fifty four sera from patients with clinical diagnosed dengue hemorrhagic fever were utilized for antibody detection by indirect immunofluorescent antibody (IFA) technique with dengue antigen prepared from LLC-MK2 cell culture. The results revealed there were at least 2 groups of patientssera reacted with dengue viral antigens of serotype 1,2,3 and 4 as homologous or heterologous antibody. All these sera were grouped according to their stages of infection as during fever (1-5 days before shock or subsidence of fever), at the day of shock or subsidence of fever and after shock or subsidence of fever (1-210 days after). There was no correlation between IFA level and stages of infection. Thus, the finding of antibody level by IFA technique could not indicate the duration of infection. By using immunoblotting technique, all 62 sera obtained from patients who had IFA antibody, showed specific antibodies reacting with various components of dengue viral proteins. However, the antibody detection by immunoblotting technique was found into 2 patterns, one consisted of antibodies reacted with 3 major viral proteins with molecular weight (Mr) of 130, 92-98 and 54-60 KD, while the other consisted of additional reactive band at Mr 42-46 KD. The former immunoblotting pattern was usually found in patients during febrile period and the latter showed in those patients with shock or subsidence of fever and thereafter. The specificity of antibody reacting with dengue viral antigen by immunoblotting technique was also defined by immunoprecipitation. The result of the above study revealed the viral antigens captured by patientssera comprised a variety of proteins in the MW range from 42 to 130 KD as identified by immunoblotting technique. The consistent detection of antibodies reacting with viral proteins of Mr 92-98 and 54-60 KD; particularly the 92-98 KD reactive band was specific in only patientssera not in any control sera. This preliminary finding may suggest the possibility of characterization of immunoblotting pattern in correlation with clinical features of patients which may be useful for specific diagnosis of dengue infection.

Mahidol University. Mahidol University Library and Knowledge Center

Address: NAKHONPATHOM

Email: liwww@mahidol.ac.th

Name: Somnate Boonpucknavig

Role: Thesis Advisors

Name: Galayanee Doungchawee

Role: Thesis Advisors

Created: 1991

Modified: 2017-12-21

Issued: 2017-12-21

วิทยานิพนธ์/Thesis

application/pdf

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Pathobiology

Grantor: Mahidol University

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