Synergistic interaction between antibiotics and natural product extracts to inhibit methicillin-resistant staphylococcus aureus (MRSA)

การเสริมฤิทธิ์ระหว่างยาปฏิชีวนะและสารสกัดธรรมชาติในการยับยั้ง staphylococcus aureus ที่ดื้อต่อยา methicillin (MRSA)

Name: Keerati Joyjamras

ThaSH: Antimicrobial

ThaSH: Natural

Abstract: Methicillin-resistant Staphylococcus aureus (MRSA) is one of the Staphylococcus aureus strains that resists to a variety of antibiotics, especially β–lactam antibiotics. The treatment of MRSA infections are very difficult because very few antibiotics are effective against MRSA. Therefore, the objectives of this research were to search for potential antimicrobial agents from native Thai plant extracts and to study the synergism between these plant extracts and antibiotics against MRSA. Eleven native Thai plants, i.e., Acanthus ebracteatus Vahl., Cissus quadrangularis Lin, Kaempferia parviflora, Barleria lupulina Lindl, Orthosiphon aristatus (Blume) Miq, Glycyrrhiza glabra L., Stevia rebaudiana Bertoni, Clitoria ternatea Linn., Curcuma longa L.,Curcuma zedoaria (Berg.) Rosc. and Zingiber cassumunar Roxb were extracted with water and 95% ethanol. These extracts were determined for antimicrobial activities against S.aureus by agar-well diffusion method. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were also determined. The synergistic effect between crude plant extracts and antibiotics was assayed using disc diffusion technique. In addition, the interaction between crude plant extracts and antimicrobial agents was estimated by calculating the fractional inhibitory concentration (FIC index) of the combination. The results showed that Kaempferia parviflora ethanol extract possessed antibacterial activity against only MRSA with inhibition zones of 15+3 mm. The MIC and MBC of Kaempferia parviflora ethanol extract against MRSA was 46.87 μg/mL and 96.75 μg/mL, respectively. Study of synergism between plant extracts and antibiotics revealed that when Kaempferia parviflora ethanol extract was combined with cefoxitin, the MIC and MBC of cefoxitin against MRSA reduced from 30.47±11.32 μg/mL to 1.76±0.62 μg/mL and from 89.06±14.82 μg/mL to 3.74±1.21 μg/mL, respectively. In addition, the FIC of this combination indicated that Kaempferia parviflora ethanol extract and cefoxitin could be synergistic in antibacterial effect against MRSA.

Rangsit University Library

Address: Pathumthani

Email: rattanapornn@rsu.ac.th

Name: Acharawan Thongmee

Role: Advisor

Created: 2014

Modified: 2559-06-14

Issued: 2016-06-14

วิทยานิพนธ์/Thesis

application/pdf

eng

DegreeName: Master of Science

Descipline: Biomedical Sciences

Grantor: Rangsit University

Level: Master's Degree

©copyrights Rangsit University

ลำดับที่.	ชื่อแฟ้มข้อมูล	ขนาดแฟ้มข้อมูล	จำนวนเข้าถึง	วัน-เวลาเข้าถึงล่าสุด
1	01-Title.pdf	1018.12 KB	19	2019-08-14 06:18:45
2	02-Acknowledge.pdf	34.61 KB	10	2025-09-26 13:34:31
3	03-Abstract.pdf	90.1 KB	8	2019-08-14 06:19:48
4	04-Content.pdf	72.11 KB	7	2022-06-20 14:29:14
5	05-Chapter 1.pdf	70.02 KB	16	2022-06-20 14:26:23
6	06-Chapter 2.pdf	873.55 KB	25	2025-09-26 13:34:42
7	07-Chapter 3.pdf	171.9 KB	6	2022-06-20 14:27:17
8	08-Chapter 4.pdf	620.53 KB	9	2022-06-20 14:28:35
9	09-Chapter 5.pdf	76.86 KB	14	2022-06-20 14:28:45
10	10-Bibliography.pdf	109.42 KB	4	2019-08-14 06:23:21
11	11-Appendix.pdf	35.2 KB	4	2019-08-14 06:23:44
12	12-Profile.pdf	36.79 KB	9	2019-08-14 06:24:04

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