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Nyo Mi Swe. Spectral characteristics of ethambutol-copper (II) ion complex and its application for quantitative analysis. Master's Degree(Pharmaceutical Chemistry). Mahidol University. : Mahidol University, 2007.
Spectral characteristics of ethambutol-copper (II) ion complex and its application for quantitative analysis
Name: Nyo Mi Swe
LCSH:
Copper
MeSH:
Graphite
MeSH:
Ethambutol
ThaSH:
Tablets
-- chemical analysis
Abstract:
Ethambutol dihydrochloride is a bacteriostatic antimicrobial agent used as a
first-line defense against tuberculosis. Ethambutol acts as a chelating agent that
disrupts one of several metal-containing enzyme systems in the nucleic acid
structure of mycobacteria. Since ethambutol-copper (II) ion complex shows
characteristic absorption, this chelating property can be used for quantitative
analysis of ethambutol by complexation. The studies of the effect of pH and time on
the complexation showed that the complex was formed immediately and stable with
maximum absorption in UV region at 260 nm at pH 7. The stoichiometry of
ethambutol-copper (II) ion complex, determined by Jobs continuous variation
method with UV spectrophotometry, was 1:2. The other spectral characteristic of
the complex such as circular dichroism spectral property was also determined.
Based on the UV spectral character of the complex, ion-pair reversed phase
chromatographic methods were developed for quantitative analysis of ethambutol in
pharmaceutical formulations. Chromatographic conditions, using C18 column and
porous graphite carbon column, tetrahydrofuran as organic solvent, and sodium-1-
octane sulphonate as an ion pairing agent, were optimized through the study of the
effects of mobile phase composition, copper(II) ion and reagent concentrations,
flow rate and column temperature.
Three simple, inexpensive, precise and accurate UV spectrophotometric and
HPLC methods for determination of ethambutol in drug formulations were
developed by complexation with copper (II) ion. Spectrophotometric, HPLC with
C18 column and HPLC with graphite column methods showed linearity over the
concentration range of 7.537.5, 6.0250.0 and 3.090.0 μg/mL with correlation
coefficient of 0.9998, 0.9999 and 0.9997, respectively. These methods were applied
for quantitative analysis of ethambutol in tablets. It was found that there were no
statistically significantly differences between the ethambutol amounts indicated by
both HPLC methods (P = 0.01).
Mahidol University
Address:
NAKHON PATHOM
Email:
liwww@mahidol.ac.th
Name: Pisamai Kulkanjanatorn
Role:
Thesis Advisors
Created:
2007
Modified:
2553-06-19
Issued:
2010-06-09
วิทยานิพนธ์/Thesis
application/pdf
CallNumber:
TH N997s 2007
eng
DegreeName: Master of Science
Level: Master's Degree
Descipline: Pharmaceutical Chemistry
Grantor: Mahidol University
©copyrights Mahidol University
RightsAccess:
ใช้เวลา
0.422025 วินาที
Nyo Mi Swe
| Title | Contributor | Type |
|---|---|---|
| Spectral characteristics of ethambutol-copper (II) ion complex and its application for quantitative analysis
มหาวิทยาลัยมหิดล Nyo Mi Swe | Pisamai Kulkanjanatorn | วิทยานิพนธ์/Thesis |
Pisamai Kulkanjanatorn
| Title | Creator | Type and Date Create |
|---|---|---|
| Determination of ciprofloxacin in drug formulation by complexation with metal ion
มหาวิทยาลัยมหิดล Pisamai Kulkanjanatorn | Sununta Lojanapiwat | วิทยานิพนธ์/Thesis |
| Spectral characteristics of ethambutol-copper (II) ion complex and its application for quantitative analysis
มหาวิทยาลัยมหิดล Pisamai Kulkanjanatorn | Nyo Mi Swe | วิทยานิพนธ์/Thesis |