Supachai Worrasinchai. Application of B-glucan prepared from spent brewer's yeast as a fat replacer in mayonnaise . Master's Degree(Biotechnology). Mahidol University. : Mahidol University, 2004.
Application of B-glucan prepared from spent brewer's yeast as a fat replacer in mayonnaise
Abstract:
Application of β-glucan prepared from spent brewer’s yeast as a fat replacer in
mayonnaise was studied. Fat was partially substituted by β-glucan at levels of 25, 50,
and 75% which were referred to as 25B, 50B, or 75B formulations, respectively. The
full fat (FF) (100% oil) mayonnaise without β-glucan substitution was used as a
control experiment. Physicochemical, rheological, microbiological analyses, and
sensory evaluation of FF and reduced fat (RF) mayonnaises were performed. The
results indicated that all RF mayonnaises had significantly lower fat and energy
contents but higher water content than their FF counterpart; these differences
increased with increasing substitution levels of β-glucan. With regard to pH, there
were no significant differences between FF and RF mayonnaises after one day of
storage. However, pH values of RF samples decreased with an increasing degree of
substitution after 2 months storage. In terms of texture, 50B and 75B formulations
showed similar firmness and adhesiveness values as those of the FF sample.
Microstructure analysis showed close packing structures of large droplets for FF and
25B and loose structures of a network of aggregated small droplets for 50B and 75B
samples. Both FF and RF mayonnaises exhibited thixothopic shear thinning behaviour
under steady shear tests and were rheologically classified as weak gels under small
amplitude oscillatory shear tests. Microbial loadings of all mayonnaises tested were in
the acceptable limit throughout the storage time. The RF mayonnaises exhibited
higher storage stability than the FF sample. Sensory evaluation demonstrated that
mayonnaises substituted with β-glucan of not more than 50% were acceptable. This
study shows good potential for spent brewer’s yeast β-glucan to be used as a fat
replacer in mayonnaise.