Natural variation of high light tolerant phenotype in the green alga chlamydomonas reinhardtii

Name: Nittaya Suwannachuen

LCSH: Kasetsart University -- Theses. M.S. (Genetics) 2022

Classification :.LCCS: Genetics

LCSH: Kasetsart University. -- Department of Genetics -- Theses

LCSH: Chlamydomonas reinhardtii

LCSH: Green algae

LCSH: Oxidative Stress

LCSH: Phenotype

LCSH: Photosynthesis

Abstract: Photosynthetic organisms required light for survive, but absorption of too much light leading to photo-oxidative damage to the photosynthetic machinery, photoinhibition, and cause program cell death if they cannot alleviate the damage caused by high light stimulation. To study natural variation and investigating proteins that are involved in adaptation and acclimation within a species with different phenotypes can help in revealing pathways and proteins that play an important role in high light tolerance. However, studies in plants are still inconclusive due to differences in cell types, tissue layers, culture techniques, and stress regimes used. Chlamydomonas reinhardtii, a unicellular green alga, has also been used as a model organism to study photosynthesis and photoprotection. Nonetheless, the majority of research has been conducted with strains that have been consistently utilized in the laboratory and originated from the same source. Despite the availability of many field isolates of this species, very few studies have compared light responses of field isolates. This study examined the responses of two field isolates of Chlamydomonas to high light stress. The light tolerant strain, CC-4414, managed reactive oxygen species (ROS) slightly better than the sensitive strain, CC-2344. The proteomic data indicated that most proteins expressed in the high light tolerance phenotype related to membranes proteins. The morphology of cells under light stress revealed that this strain utilized the formation of palmelloid structures and a small degree of cell aggregation to shield the cells from excessive light. As indicated by proteomic data, morphological modifications occur simultaneously with the increase of protein degradation and autophagy. By protecting cells from stress, cells are able to continue to upregulate ROS management mechanisms and prevent cell death.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Anchalee Sirikhachornkit

Role: Thesis Advisor

Name: Jaruswan Warakanont

Role: Thesis CO-Advisor

Created: 2022

Modified: 2025-07-24

Issued: 2025-07-24

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2565/nittaya-suw-all.pdf

CallNumber: QK569.C486 .N58

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Genetics

Grantor: Kasetsart University

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