Development of unicellular microalgae for carotenoid production

Name: Thanaporn Intha

LCSH: Kasetsart University -- Theses. M.S. (Genetics) 2022

Classification :.LCCS: QK569.C486

LCSH: Kasetsart University. -- Department of Genetics -- Theses

LCSH: Chlamydomonas reinhardtii

LCSH: Chlamydomonas

LCSH: Microalgae.

LCSH: Green algae

LCSH: Carotenoids

LCSH: Zinc oxide

LCSH: Unicellular organisms

Abstract: Microalgae produce beneficial bioactive substances, especially pigments such as chlorophylls and carotenoids that are used in a variety of industries, including cosmetics, pharmaceuticals, and other bio-based products. The production of these pigments can be combined with bioremediation applications such as wastewater treatment. Microalgae can absorb and accumulate various contaminants, including heavy metals and certain toxin-containing organic compounds. Utilizing agricultural and industrial wastewater for algae cultivation is therefore a cost-effective alternative method of producing high-value compounds. The agriculture sector can result in the contamination of effluent with herbicides. Norflurazon is a common herbicide utilized frequently for weed management. The presence of norflurazon renders water unusable and necessitates its remediation. ZnO nanoparticles (ZnO NPs) are utilized in a variety of industrial productions of numerous consumer products. Contaminated water with ZnO NPs may pose threats to human health and the environment. To explore the possibility of using microalgae for pigment production using wastewater, twenty strains of the widely used model organism Chlamydomonas reinhardtii, a unicellular green microalga, were investigated for their response to norflurazon and ZnO. Norflurazon at 10 µM had a higher inhibitory effect than ZnO NPs at 200 mg L-1 on growth and pigment production. Norflurazon induces oxidative stress in cells, resulting in photosystem damage and decreased pigment production, with the exception of the norflurazon resistant strain CC-1009, which displayed increased cell density and pigment content. ZnO NPs, on the other hand, did not cause photosystem damage, but the aggregation of ZnO NPs disrupted cellular function and structure, resulting in organelle damage. In addition, the physiological responses of Chlamydomonas strains differed based on dose and exposure time to each chemical, providing support for the use of natural algal strains for specific types of environmental pollutants.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Anchalee Sirikhachornkit

Role: Thesis Advisor

Name: Jaruswan Warakanont

Role: Thesis CO-Advisor

Created: 2022

Modified: 2025-07-24

Issued: 2025-07-24

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2565/thanaporn-int-all.pdf

CallNumber: QK569.C486 .T43

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Genetics

Grantor: Kasetsart University

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