Function of essential oils against the storage fungi and its application for maize grain storage

Name: Sansern Rangsuwan

LCSH: Kasetsart University -- Dissertations. Ph.D. (Plant Pathology) 2021

Classification :.LCCS: QK625.M7

LCSH: Kasetsart University. -- Department of Plant Pathology -- Dissertations

LCSH: Aspergillus flavus

LCSH: Antifungal agents

LCSH: Essences and essential oils -- Utilization

LCSH: Fungal diseases of plants

LCSH: Grain -- Storage

Abstract: Aspergillus flavus can often found as a contaminant in agricultural products. In this study, we isolated some of the A. flavus fungi from maize grains and have grouped them into two categories of atoxigenic and toxigenic strains concerning the colony morphology, the molecular confirmation with ITS1-5.8s-ITS2 region, and the aflatoxin produced. Both strains appeared similar in morphology characteristics, except one group produced an abundance of sclerotia and highly aflatoxin produced. The essential oils major compounds of clove oil are eugenol; cinnamon oil as benzyl cinnamate, and lemongrass oil as trans-citral. Through contact assay, three essential oils have the antifungal potential to control soil fungi and storage fungi mycelium growth. Clove and cinnamon oil showed better control over both fungal groups under the fumigation assay of essential oils in modified atmosphere conditions. For the testing with atoxigenic and toxigenic A. flavus, the sensitive colonial response was seen with clove oil in the contact and fumigation assay. It was found that the fungal colony at higher dose did not produce sclerotia, small vesicle or phialide of the tested fungi. The functional of the target essential oils was tested to a toxigenic A. flavus biochemical characteristics. It was showed the increased electro conductivity in treatment and reduction of the ergosterol content from fungal mycelium and the aflatoxin content in culture broth. The expression of genes; erg3, erg6, erg11 and erg28 were involved the ergosterol biosynthesis pathway. They were downregulated with the clove and cinnamon oil tested sample in the contact assay. An application of both essential oils suspension at concentration 200 l/kg was tested to 5 kg of maize grain and packed in polypropylene bag. The spraying of essential oil result on maize grain after 30 days incubation, they can reduce percentage contaminated severity index and the aflatoxin accumulation in maize grain. The residue of essential oils constituents was analyzed and benzyl cinnamate was observed until 15 days. This means the spraying of clove and cinnamon oil showed efficiency in applying to the storage grain in practice.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Chainarong Rattanakreetakul

Role: Thesis Advisor

Name: Ratiya Pongpisutta

Role: Thesis CO-Advisor

Name: Jutamas Romkaew

Role: Thesis CO-Advisor

Created: 2021

Modified: 2025-07-12

Issued: 2025-07-12

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2564/sansern-ran-all.pdf

CallNumber: QK625.M7 .S26

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degraee

Descipline: Plant Pathology

Grantor: Kasetsart University

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