Comparative phytochemistry analysis within the genus Clausena (Rutaceae) and its bioactive compounds

Name: Natthawadi Wongthet

LCSH: Kasetsart University -- Dissertatoins. Ph.D. (Botany) 2018

Classification :.LCCS: QK495.R98

LCSH: Kasetsart University. -- Department of Botany -- Theses

LCSH: Rutaceae

LCSH: Botanical chemistry

LCSH: Plant extracts

LCSH: Bioactive compounds

Abstract: Three Clausena species (Rutaceae), 57 individuals were collected from various floristic regions of Thailand during 2015 to 2018. Different plant parts (leaves, stem bark and root bark) of the samples were analyzed comparatively by using chromatographic techniques i.e. HPLC and TLC. Therefore, fourteen compounds were isolated from Clausena excavata (NW18) and C. wallichii (NW36), from which eight compounds are coumarins, four carbazole alkaloids, and two phenylpropanoids by using column chromatography, medium pressure liquid chromatography (MPLC), size exclusion chromatography and preparative thin-layer chromatography. Structure elucidation confirmed that one of these phenylpropanoids is not yet described in literature and named as 3-hyderoxy-dictagymin. The chemical structures of all isolated compounds were elucidated through extensive analyses of their 1H and 13C NMR. The results of comparative analyses of all collected samples suggests that Clauslactone E may be regarded as a chemical marker for C. excavata. Furthermore, the antibacterial activity of lipophilic extracts and three compounds, i.e. clausenidin, nordentatin and dentatin were evaluated using disk diffusion method and broth microdilution against four Gram positive and eight Gram negative human pathogenic bacterial strains. The lipophilic stem bark extracts of Clausena three species revealed prominent effects on the Gram positive strains Staphylococcus aureus (ATCC 25923 and ATCC 43300), E. faecium UCLA192, and Enterococcus faecalis ATCC 29212. Dentatin demonstrated the highest activity against Stenotrophomonas maltophila DMST 19079 and Acinetobacter baumannii ATCC 19606 with an MIC of less than 16 and 64 µg/mL, respectively. The effects of dentatin on changes of the cell morphology of bacteria were also examined by using scanning electron microscopy (SEM).

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Srunya Vajrodaya

Role: Thesis Advisor

Name: Nuttha Sanevas

Role: Thesis CO-Advisor

Name: Johann Schinnerl

Role: Thesis CO-Advisor

Created: 2018

Modified: 2025-07-03

Issued: 2025-07-03

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2561/natthawadi-won-all.pdf

QK495.R98 .N38

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degraee

Descipline: Botany

Grantor: Kasetsart University

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