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Sugunya Maneenin. Development of oral vaccination method for preventing streptococcosis disease in nile tilapia (Oreochromis niloticus Linn) by using algae as a delivery system. Master's Degree(Biochemistry). Kasetsart University. Office of the University Library. : Kasetsart University, 2018.
Development of oral vaccination method for preventing streptococcosis disease in nile tilapia (Oreochromis niloticus Linn) by using algae as a delivery system
Name: Sugunya Maneenin
Classification :.LCCS: SH179.N54
LCSH:
Nile tilapia
-- Diseases
LCSH:
Streptococcus
LCSH:
Oral vaccines
LCSH:
Algae
-- Utilization
Abstract:
Streptococcus agalactiae is the major infectious bacterial of streptococcosis disease in many fish species especially Nile tilapia (Oreochromis niloticus). Although multiple types of vaccines were developed and used to control Streptococcosis disease, the principal method for vaccine application in fish is based on injection method. However, stress introduction to injected animals and skill labor requirement are the major drawback of this method. Hence, in this study, the candidate antigen for subunit vaccine will apply for oral vaccination system by using Chlamydomonas reinhardtii to mediate transportation of subunit vaccine to the fish. The surface immunogenic protein (Sip protein) of S. agalactiae was used as potential universal vaccine. For expression of Sip protein in microalgaes chloroplast, codon usage of gene should be optimized. The sequence of Sip gene was optimized to be compatible with the C. reinhardtii codon usage table from Kasuza database by using bioinformatics. The sequence of optimized Sip (tSip-op) gene contains 1,042 bp of nucleotides sequence and 343 of amino acid residues. The wild type and optimized Sip gene were inserted into pASapI vector and introduced to algal cell by glass bead transformation to construct transgenic C. reinhardtii. The result indicated that the both gene was successfully constructed and completely integrated to C. reinhardii chloroplast genome. The SDS-PAGE and western blot presented an overexpress protein in algal transformant. In addition, the in vitro digestion showed that algal cell could be digested in digestive system that had potentially used as a vaccine delivery system for orally administration. Hence, these system will be solved for many disadvantage of route vaccination. It will be useful in the application to prevent the other disease in aquatic animals.
Kasetsart University. Office of the University Library
Address:
Bangkok
Email:
tdckulib@ku.ac.th
Name: Sasimanas Unajak
Role:
Thesis Advisor
Name: Nontawith Areechon
Role:
Thesis CO-Advisor
Created:
2018
Modified:
2025-07-02
Issued:
2025-07-02
วิทยานิพนธ์/Thesis
application/pdf
URL: https://www.lib.ku.ac.th/KUthesis/2561/sugunya-man-all.pdf
CallNumber:
SH179.N54 .S84
eng
DegreeName: Master of Science
Level: Master's Degree
Descipline: Biochemistry
Grantor: Kasetsart University
©copyrights Kasetsart University
RightsAccess:
ใช้เวลา
0.034039 วินาที
Sugunya Maneenin
| Title | Contributor | Type |
|---|---|---|
| Development of oral vaccination method for preventing streptococcosis disease in nile tilapia (Oreochromis niloticus Linn) by using algae as a delivery system
มหาวิทยาลัยเกษตรศาสตร์ Sugunya Maneenin | Sasimanas Unajak Nontawith Areechon | วิทยานิพนธ์/Thesis |
Sasimanas Unajak
Nontawith Areechon