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Hadsaya Panyanitikoon. Genetic diversity using RAPD markers and linkage map construction of cucumber (Cucumis sativus L.). Master's Degree(Genetics). Kasetsart University. Office of the University Library. : Kasetsart University, 2017.
Genetic diversity using RAPD markers and linkage map construction of cucumber (Cucumis sativus L.)
Name: Hadsaya Panyanitikoon
Classification :.LCCS: QK495.C96
LCSH:
Gene mapping
LCSH:
Genetic markers
Abstract:
The objectives of this study are to evaluate the cucumber genetic control of downy mildew (DM) disease in resistant and susceptible cucumber lines and also to construct cucumber genetic linkage map using F2 population segregating for downy mildew resistance. For the first objective, 38 cucumber accessions (25 are DM resistant and 13 are DM susceptible) were screened for genetic diversity using 26 RAPD markers. From the RAPD band pattern scoring, the percentage of polymorphic bands of each primer ranged from 57.14 100 %. PIC value ranged from 0.02 - 0.48 with the average of 0.27. A RAPD data matrix was used to create a UPGMA dendrogram. The 38 cucumber accessions were separated into two main groups depending on both downy mildew disease resistance and location of origin of cucumber except accession PI212985, PI217644 and PI504566 that formed a subgroup separating from the two main groups. For the second objective, using microsatellite markers, a genetic linkage map of cucumber was constructed with a population consisting of 315 F2 individuals derived from a cross of the two cucumber lines, CSL0067 (DM resistant) and CSL0139 (DM susceptible). The constructed linkage map using 51 microsatellite loci, showed presence of eleven linkage groups and spanning 528.6 cM in length with an average marker interval of 10.4 cM between two neighboring loci. Information received from this thesis is crucial for further cucumber germplasm management and for quantitative loci identification in the future.
Kasetsart University. Office of the University Library
Address:
Bangkok
Email:
tdckulib@ku.ac.th
Name: Sompid Samipak
Role:
Thesis Advisor
Role:
Thesis Co-Advisor
Created:
2017
Modified:
2023-06-16
Issued:
2023-06-16
วิทยานิพนธ์/Thesis
application/pdf
URL: https://kasetsart.idm.oclc.org/login?url=https://www.lib.ku.ac.th/KUthesis/2560/hadsaya-pan-all.pdf
CallNumber:
QK495.C96.H33
eng
DegreeName: Master of Science
Level: Master's Degree
Descipline: Genetics
Grantor: Kasetsart University
©copyrights Kasetsart University
RightsAccess:
ใช้เวลา
0.01801 วินาที
Hadsaya Panyanitikoon
| Title | Contributor | Type |
|---|---|---|
| Genetic diversity using RAPD markers and linkage map construction of cucumber (Cucumis sativus L.)
มหาวิทยาลัยเกษตรศาสตร์ Hadsaya Panyanitikoon | Sompid Samipak Chatchawan Jantasuriyarat | วิทยานิพนธ์/Thesis |
Sompid Samipak
| Title | Creator | Type and Date Create |
|---|---|---|
| Genetic evaluation of downy mildew resistant germplasm and identificatiion of QTLs conferring downy mildew resistance in cucumber (Cucumis sativus L.)
มหาวิทยาลัยเกษตรศาสตร์ Chatchawan Jantasuriyarat ;Sompid Samipak ;Arinthip Thamchaipenet | Pawinee Innark | วิทยานิพนธ์/Thesis |
| Genetic diversity using RAPD markers and linkage map construction of cucumber (Cucumis sativus L.)
มหาวิทยาลัยเกษตรศาสตร์ Sompid Samipak ;Chatchawan Jantasuriyarat | Hadsaya Panyanitikoon | วิทยานิพนธ์/Thesis |
Chatchawan Jantasuriyarat